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Name: guest11
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Date: Around 1993


Question:
A stage micrometer can be used to measure specimens observed under the microscope. Is this done by measuring the field of view in each objective? And then using these distances to estimate the size of the specimen?



Replies:
Usually a stage micrometer, that is a calibrated set of marks on a slide that can be viewed through a microscope, is used to calibrate the scale on a micrometer that inserts into the ocular (eyepiece) of the microscope. Then to measure you simply count the divisions in the ocular micrometer which are superimposed on your view of the specimen and use the calibration factor to calculate the actual dimension. There are fancy (translate expensive) stages that move in calibrated amounts that can do the same thing. You can also make very accurate measurements by making a video image and digitizing it for use with computer image analysis software.

moodywj



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