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Nucleotide sequencing
Name: jcowan
Status: N/A
Age: N/A
Location: N/A
Country: N/A
Date: Around 1993
Question:
We have been studying DNA. If no one has ever seen a sequence of
nucleations, how do scientists really know they are there?
Replies:
What it boils down to is that it is the best explanation for the
evidence. That evidence includes electron micrographs of DNA. Even with
this, DNA only looks like a bumpy line. There is more though. Remember that
sequences of nucleations include both DNA and RNA. When Watson and Crick came
up with their model they had to explain the evidence that was available. This
included knowing what chromosomes looked like, that there are only 4 types of
nucleations in DNA/RNA, that there must be a way for the material to reproduce
itself. With balls and sticks they could build models of what the nucleation
look like and the position of the most likely areas for bonding between them.
The sequential model makes sense of the data. Once the hypothesis was formed
they were able to predict that there is cellular machinery which does the work
of reproduction. Much of that protein machinery has been discovered and
described, this also provides more evidence that the theory is correct.
psych
After scientists knew for sure that DNA played a central role in
heredity, some set out to determine the exact structure of DNA. In 1953,
James Watson and Francis Crick succeeded in finding a reasonable DNA
structure. They worked from two kinds of clues:
1) X-Ray diffraction data, supplied by Maurice Wilkins. Wilkins fired X-rays
at DNA fibers, and recorded the way the fibers scattered (or diffracted) the
X-rays onto photographic film. The resulting photos looked like a wild
scattering of spots. The angles and positions of the spots gives information
about the position of atoms or group of atoms in the DNA. This is a very
difficult experiment and the interpretation of the spot scatters requires
extensive calculations and analysis. The data suggested that DNA is long,
skinny, has two similar parts running parallel to one another, and helical, as
well as other clues. But it was Watson and Crick who pieced all the
information together like a puzzle to suggest a three dimensional model.
2) Chargaff's observations that the total amount of pyrimidine nucleations (T
& C) always equalled the amount of purine nucleations (A & G) and that the
amount of T = A, and G = C, but that the amount of T + A does not necessarily
equal G + C. So, like good detectives, Watson and Crick put all the pieces
together. In a way, we really have "seen" nucleations!
wizkid
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Update: June 2012
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