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Name: Latasha
Status: Student
Grade:  Other
Location: VA
Country: United States
Date: Spring 2010

Why is the Gram stain technique more valuable than the simple stain technique in the diagnostic laboratory? Under what circumstances might the simple stain be preferable to the Gram stain technique?

The gram stain has two dyes. There are two types of bacterial cell walls-gram positive which is very thick and gram negative which isn't as thick. The gram positive wall holds onto crystal violet for longer than gram negative which can be destained. Then the gram negative are counterstained with a second dye. This allows you to break all bacteria down into two basic groups and allows to begin the identification process. Simple staining only uses one dye. If all you want to know is what shape the bacteria is or if there is bacteria at all, a simple stain is quicker to do.


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