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Name: Ryan
Status: Other
Grade:  Other
Location: MI
Country: United States
Date: Winter 2009 - 2010


Question:
If you have two containers of multiple strands unknown cDNA, is there a way to weed-out all of the cDNA that the containers have in common and leave only the unique strands intact?



Replies:
Some context would help on this -- what are you trying to achieve, and what materials are you working with? Is this a 'hypothetical' question or are you trying to actually do this?

I imagine there are lots of possible routes to achieving this: a combination of synthesis/probes, hybridization and/or chromatography, and PCR all might probably get the job done. This would take some work to develop and optimize a protocol, and would depend on the nature of the DNA in question.

Again, some context here (not only your project, but also your grade level and what lab facilities to which you have access) would help.

If you're asking for some one-step "magic bullet" that works generally, I don't have that.

Hope this helps,
Burr



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