Agar Pouring Temperature
Country: United States
Date: February 2006
Why must the temperature of the agar be no lower
than 45 C and no higher than 50 C when being poured into the plates?
Below 45 the agar begins to solidfy and above 50 the bacteria can be
killed. If you are adding anything to the media, such as antibiotics, etc.,
some of these can be inactivated at temps higher than 50.
I know of no reason to not pour at higher than 50 C, pouring at lower than
certain temperatures can cause lumpiness
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Update: June 2012