Country: United States
Date: September 2005
Hello, basically I have two related questions.
1) I am working with Planaria in school(standard cut to see if they grow
back stuff). Part of the assignment allows for us to run our own
experiment and during class the teacher commented on how it is debated
whether or not the worms regenerate along the cut line or if there is
some reserve of undifferentiated cells in its body that migrate to the
cut. Is it known where/how they regenerate?
2) I have thought of three different experiments which both require
dyeing a living worm. The 1st one would be to dye the worm at the cut
point and see if the line moves forward as the worm regen or if the line
stays in place. 2nd dye the worm several different colors (bands of
colors) to see if it's possible to locate cells that migrate to the wound
(I would think this would be difficult both dying multiple colors and
actually seeing dyed cells that moved) 3rd dyeing a specific tissue type
(such as nerves or skin) I think nerve tissue would be the best candidate
as long as it divides relatively slow.
For all these I would need a dye that would show up under a light
microscope and would not kill the worm or retard growth. Is there such a
This is a very difficult project. I don't dyes would work because they will
diffuse too much and they would have to be injected intra-cellularly.
Using a search engine, search under "planaria regeneration stem cells"
Ron Baker, Ph.D.
Click here to return to the Molecular Biology Archives
Update: June 2012