Country: United States
Date: July 2005
Why use 44.5 degrees celsius to maintain agar in its
Anything lower will cause agar to solidfy, and at higher temps most
bacteria are killed.
Below 44.5 deg C, the agar starts to solidify and will not pour
into a Petri plate and form a smooth (flat) surface.
Ron Baker, Ph.D.
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Update: June 2012