Hello! What is the best method for inoculating sunflower
plants with Agrobacterium tumefaciens? Any info would be greatly
Use a sterile toothpick or a pin to pick some colonies of bacteria. Then
punch the tip with the bacteria into the stem about half way through. You
don't want to push the tip through the stem. It will take about 10 days to
see a gall (tumor) form. You might want to use a permanent marker to circle
the place on the stem where you inoculated it.