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Name: Amanda
Status: Student
Age: 15
Location: N/A
Country: N/A
Date: December 2002

Hello! What is the best method for inoculating sunflower plants with Agrobacterium tumefaciens? Any info would be greatly appreciated. Thanks!

Use a sterile toothpick or a pin to pick some colonies of bacteria. Then punch the tip with the bacteria into the stem about half way through. You don't want to push the tip through the stem. It will take about 10 days to see a gall (tumor) form. You might want to use a permanent marker to circle the place on the stem where you inoculated it.


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