Microbial Ecology Protocol
Name: Roseann W.
My AP Biology students are working on a study of
microbial ecology in abandoned mine drainage waters. Does a particular
protocol exist for isolating individual species? We are getting major
biotechnology equipment within the next two weeks and I was wondering if
there were any DNA studies we could do to help us in the identification
process. Any help would be appreciated.
I am not sure there is any rapid or specific "kit" for such isolations but I
doubt it since these are not an object of routine study. I would do two
things. Consult with a microbiologist ate either a local college or at your
state Extension agency, and two consult Bergeys Manual of Systematic
Bacteriology and find the appropriate medium and temperatures (and possibly
gas concentrations) they like and do a standard streak plate followed by
pure tube cultures.
Peter Faletra Ph.D.
Office of Science
Department of Energy
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Update: June 2012