I am trying to get microalgae to grow in saline water. What is
the best way for me to provide a CO2 source to the algae? I have a CO2
gas bubbler now, but should I use another source instead?
A CO2 bubbler should work fine. Unless you're planning to grow it very
dense you don't even need a bubbler. If you get too zealous with bubbling
you might lower the pH- marine algae normally grow in somewhat alkaline
water (pH 7.5-8) so unless you're using filtered seawater you may need to
add a bit of bicarbonate/carbonate to get the pH balanced and buffered.
http://ccmp.bigelow.org is a research institute that grows and maintains
many stocks of algae. They have a listing of references/recipes for various
culture media- many of them are based on filtered natural seawater, but
some synthetic ones ( e.g. YBC-II medium) made from scratch using freshwater.
Click here to return to the Botany Archives
Update: June 2012