Department of Energy Argonne National Laboratory Office of Science NEWTON's Homepage NEWTON's Homepage
NEWTON, Ask A Scientist!
NEWTON Home Page NEWTON Teachers Visit Our Archives Ask A Question How To Ask A Question Question of the Week Our Expert Scientists Volunteer at NEWTON! Frequently Asked Questions Referencing NEWTON About NEWTON About Ask A Scientist Education At Argonne Growing Microalgae
Name: Leslie
Grade: N/A
Location: N/A
Country: N/A
Date: N/A

I am trying to get microalgae to grow in saline water. What is the best way for me to provide a CO2 source to the algae? I have a CO2 gas bubbler now, but should I use another source instead?


A CO2 bubbler should work fine. Unless you're planning to grow it very dense you don't even need a bubbler. If you get too zealous with bubbling you might lower the pH- marine algae normally grow in somewhat alkaline water (pH 7.5-8) so unless you're using filtered seawater you may need to add a bit of bicarbonate/carbonate to get the pH balanced and buffered. is a research institute that grows and maintains many stocks of algae. They have a listing of references/recipes for various culture media- many of them are based on filtered natural seawater, but some synthetic ones ( e.g. YBC-II medium) made from scratch using freshwater.


Click here to return to the Botany Archives

NEWTON is an electronic community for Science, Math, and Computer Science K-12 Educators, sponsored and operated by Argonne National Laboratory's Educational Programs, Andrew Skipor, Ph.D., Head of Educational Programs.

For assistance with NEWTON contact a System Operator (, or at Argonne's Educational Programs

Educational Programs
Building 360
9700 S. Cass Ave.
Argonne, Illinois
60439-4845, USA
Update: June 2012
Weclome To Newton

Argonne National Laboratory